FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1784882
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31330.17552981093428024No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22020.12336950005658637No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51900.043.6544081
TGGTATC13850.043.5734182
GTGGTAT14550.042.069541
TATCAAC59200.038.3356322
TCAACGC60900.037.1248934
CAACGCA60950.037.094445
ATCAACG62850.036.040673
AACGCAG64950.034.940816
GGTATCA25650.032.9787331
CGCAGAG69100.032.9634978
ACGCAGA69600.032.6656347
GCAGAGT77950.029.2755289
CAGAGTA53600.024.458910-11
AGAGTAC51050.022.14279710-11
GAGTACG63000.020.57339112-13
GTATAGG4000.019.1284941
AGTACGG59650.018.8791312-13
GTACGGG67950.018.85630414-15
GTATTAG6600.018.6776521
GTACATA2755.013135E-918.5488411
Produced by FastQC (version 0.11.5)