FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1861075
Sequences flagged as poor quality0
Sequence length91
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20650.11095737678492269No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51550.044.520181
TATCAAC56300.040.230212
GGTATCA24650.039.6554371
GTGGTAT12850.039.0274931
ATCAACG58600.038.578693
TCAACGC58850.038.4148064
TGGTATC12700.038.144732
CAACGCA59650.037.89965
AACGCAG63900.035.4453966
ACGCAGA66200.034.212997
CGCAGAG68050.033.221338
GCAGAGT77100.029.4320379
CAGAGTA57700.024.85634410-11
AGAGTAC55400.021.63110210-11
GAGTACG66000.021.6019912-13
TATACGC1000.003158724921.2472862
GTACGGG67750.020.6673814-15
AGTACGG59800.019.79106312-13
GTGTATA5550.018.37851
CCGTCTT11250.018.13515984-85
Produced by FastQC (version 0.11.5)