FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1661741
Sequences flagged as poor quality0
Sequence length91
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43400.043.295551
GGTATCA22200.038.6820141
TATCAAC48500.038.6458632
TCAACGC48600.038.4754184
ATCAACG49650.037.490553
CAACGCA50350.037.306955
AACGCAG52250.036.1130076
GTGGTAT8650.035.877111
ACGCAGA54700.034.415757
CGCAGAG56100.033.4821478
GCAGAGT60550.031.1618129
TGGTATC9700.030.671322
CAGAGTA49050.026.03510710-11
CGTCAGA3500.021.8560375
GAGTACG58350.021.73989112-13
GTACGGG58550.021.3749814-15
AGAGTAC47800.021.24828510-11
GTACTAA1708.2349015E-620.005611
AGTACGG52750.019.17380712-13
GTATAGA3359.094947E-1219.0351891
Produced by FastQC (version 0.11.5)