FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803616
Sequences flagged as poor quality0
Sequence length91
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41050.043.912021
GGTATCA19700.041.2190441
TATCAAC47400.037.5672872
TCAACGC47550.037.2679634
ATCAACG47750.037.1118663
CAACGCA48250.036.8153655
AACGCAG50250.035.3500756
ACGCAGA53500.033.201727
CGCAGAG54300.032.6342968
GTGGTAT9000.032.593981
TGGTATC9250.031.7016282
GCAGAGT61600.029.0428669
CAGAGTA50600.024.4808310-11
AGAGTAC47750.021.89272310-11
GAGTACG59900.020.39619312-13
GTACGGG62950.019.2729614-15
AGTACGG54700.019.14995212-13
CTTACAC4251.8189894E-1216.9984823
TACGGGG27150.016.90409714-15
AGAGTCG7550.016.8859085
Produced by FastQC (version 0.11.5)