FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1721073
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29780.17303159133865909No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21310.12381810649519225No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT15500.042.507021
TGGTATC15650.042.367522
GTATCAA55600.041.5131451
TATCAAC60200.038.3377082
TCAACGC62500.036.7855224
ATCAACG63250.036.349333
CAACGCA64250.036.0481575
AACGCAG68650.033.7377176
GGTATCA29900.032.8398671
ACGCAGA73250.031.6190287
CGCAGAG73550.031.547848
GCAGAGT80600.028.7883859
CAGAGTA60550.023.58222810-11
AGAGTAC57150.020.82099510-11
GAGTACG68750.020.52255212-13
GTACGGG71350.019.32827214-15
AGTACGG63800.018.41791512-13
AGAGTCG18450.017.5055965
CGACTCT2455.655038E-717.345775
GTATAGG5300.016.8424031
Produced by FastQC (version 0.11.5)