FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1631352
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46200.029.4743771
GTGGTAT9350.027.9507451
GGTATCA22550.027.0824411
TGGTATC9650.026.4994722
TATCAAC52050.025.8326952
TCAACGC52650.025.5312614
ATCAACG52850.025.2793753
CAACGCA54050.024.8699535
AACGCAG54950.024.5641756
AGAGTAC52900.024.53397611
CGCAGAG56750.023.7835888
CAGAGTA54950.023.6680110
ACGCAGA57350.023.5841437
GCAGAGT60700.022.2852789
AGTACGG59250.021.62946513
TGCCGAC5300.021.27760755
CCGACGC650.003238502421.155932
GAGTACG62350.020.59627312
TACGGGG24400.019.72406415
GTACGGG65700.019.21301514
Produced by FastQC (version 0.11.5)