FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930169
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT47200.5074346704738601No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA35540.38208110569154635No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33770.36305230554877665No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC30130.3239196318088433No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT27730.2981178689033928No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT25660.27586384839744177No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA25390.2729611500705786No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG24850.2671557534168522No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA23640.25414736461868753No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC23570.25339481320061197No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22300.23974138032981104No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA21390.2299582118948277No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAA20270.21791738920561746No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA19960.21458466149699676No Hit
CTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATT19450.20910178687958855No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAG19150.20587656651640723No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAG18110.194695802590712No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTAT18090.19448078789983325No Hit
GTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTC17540.1885678839006675No Hit
CCGCTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGAT17360.18663275168275872No Hit
GCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGAATGGGG17130.18416008273765305No Hit
CGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGC16780.18039732564727487No Hit
ATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCA16470.17706459793865417No Hit
CATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAAT16010.1721192600484428No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC15650.16824899561262524No Hit
TCTCAGTACCCTTAGGAAAAGAAATCAATTGAGATTCCCTCAGTAGCGGC15040.16169104754082322No Hit
CCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTT14930.16050846674099006No Hit
GTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGAA14820.15932588594115693No Hit
GGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAA14080.15137034237864302No Hit
GTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGAGGCG13840.14879016608809797No Hit
TCGCATGACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGT13390.14395233554332598No Hit
GTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCG13310.14309227677981098No Hit
GCAAATATCCTGTGATCCGGAGATTTCTGAATGGGGAAACCCACTTACCA13210.1420172033254172No Hit
GCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTG13000.1397595490711903No Hit
CTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGATGTT12910.13879198296223588No Hit
CCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCG12370.13298658630850954No Hit
GATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAG11940.1283637704546163No Hit
GTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAG11730.1261061162003894No Hit
GTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCG11710.12589110150951063No Hit
AATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACG11380.1223433591100112No Hit
CCCACTTACCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAG10940.1176130359106786No Hit
GTATCAACGCAGAGTACGGGTCAAGTAATTAAGTGCATGTGGTGGATGCC10150.10911995562096781No Hit
GAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCT10100.10858241889377092No Hit
TCCTAAGGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACT9910.10653977933042275No Hit
GATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATTGATTTCTTTTC9840.10578722791234711No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC14300.052.3157352
GTGGTAT14150.051.97751
GACAGGT2200.046.3782461
GTATCAA66050.045.5063861
TATCAAC74700.039.94592
TCAACGC78750.037.8294264
ATCAACG79750.037.3550723
AACGCAG80750.036.6819576
CAACGCA81300.036.5906225
GGTATCA35050.035.5390131
ACGCAGA85700.034.5136387
CGCAGAG86400.034.28328
GCAGAGT92600.032.0336729
CGTCAGA1750.031.5694925
CAGGGAC905.1562372E-528.3315944
CAGAGTA63250.027.31255510-11
TAGTAGG850.001226817424.9984667
ACTCCGC1051.470943E-424.2842258
AGATGTG2451.8189894E-1224.2842249
AGAGTAC63100.023.70694410-11
Produced by FastQC (version 0.11.5)