FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1991261
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57030.28640143105298604No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACG358.670103E-539.2856444
TGGTATC11900.031.4293042
GTGGTAT13200.029.1842081
GTATCAA49900.028.5091191
GGTATCA23300.025.3907971
GCGACGT550.001227242824.99869724
TATCAAC56400.024.9163632
ATCAACG56450.024.7394393
TCAACGC56400.024.712014
CAACGCA57450.024.213725
AGAGTAC55050.023.77654611
AACGCAG60200.023.291456
CGAACGG600.00203607222.91662445
CGCAGAG60850.022.726958
ACGCAGA62050.022.5970237
CAGAGTA58600.022.52272810
TGCCGAC7800.021.50961355
GCAGAGT66150.020.991289
AGTACGG63550.020.38155213
GAGTACG65600.019.82747512
Produced by FastQC (version 0.11.5)