FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1267343
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45560.3594922605798115No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT17300.1365060603167414No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT13400.1057330178175916No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT13100.038.2141721
TGGTATC13550.037.3377842
GTATCAA55000.027.95591
CGATAGG759.752428E-625.66701722
TATCAAC63500.024.8546832
GGTATCA27450.024.5498091
AGAGTAC64250.023.2360611
TCAACGC67800.023.1880914
ATCAACG68000.023.1630523
AACGCAG69000.022.9450596
CAACGCA69050.022.927545
CAGAGTA65950.022.7603710
ACGCAGA71700.022.1193567
CGCAGAG71500.022.06598
CCATGCG752.8787597E-421.9933559
CGATCGT650.00323819121.15580647
GCAGAGT75800.020.8182479
AGTACGG71300.020.59879513
GAGTACG72500.020.2530512
TACGGGT10550.018.24957515
Produced by FastQC (version 0.11.5)