FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527605
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21330.4042797168336161No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT9080.1720984448593171No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT6900.13077965523450308No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT6450.12225054728442679No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC5850.11087840335099175No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC5790.10974118895764823No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT5680.10765629590318514No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA5650.10708768870651339No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA5520.10462372418760246No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAG5300.10045393807867628No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6250.033.8843841
TGGTATC5950.033.7310642
TACGTTA350.00409694931.42965347
GTATCAA27950.029.7176171
TATCAAC31500.027.0567282
GGTATCA12250.026.0441841
ATCAACG33950.024.7779063
TCAACGC34000.024.7391244
AACGCAG34650.024.5200066
CAACGCA35000.024.4296075
CAGAGTA31400.024.34797310
AGAGTAC31300.024.25003611
ACGCAGA35800.023.6555487
TTACGTT701.798404E-423.5722446
CGCAGAG36250.023.134348
GCAGAGT37650.022.2783269
GCTTATA752.867295E-422.0028461
AGTACGG34500.021.36305613
GAGTACG35050.021.26321612
AAATCGA956.0500373E-520.26385520
Produced by FastQC (version 0.11.5)