FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718745
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19610.2728366806029955No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT10340.14386187034344586No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT8490.11812256085259724No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT7450.1036528949766607No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT7270.10114852972890247No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC7250.10087026692359598No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA7190.10003547850767656No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7750.032.3266071
TGGTATC8300.031.1556972
GTATCAA32700.030.8144041
CGTCCGA507.040421E-427.4925886
TATCAAC38750.026.4094242
CCGGTCT759.755655E-625.663329
TCAACGC39450.025.2938444
CAACGCA40250.025.1343335
ATCAACG40100.025.0947323
GGTATCA14850.024.8427011
AACGCAG40900.024.669396
AGAGTAC37400.024.33675811
CAGAGTA38000.024.31262210
ACGCAGA42350.023.7598297
CGCAGAG42550.023.4543118
ACTCCGG952.3194043E-623.1516536
GCAGAGT44500.022.18259
ATTACTC1658.367351E-1121.6623363
TCCGGTC904.0012535E-521.3831238
CGTGCGT650.003241031721.151099
Produced by FastQC (version 0.11.5)