FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1042536
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT96140.9221743901409639No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64440.6181081516609498No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40700.3903941926226049No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA20230.19404605692273455No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC15180.1456064826538364No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT14840.14234520438622741No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT13770.13208176983816386No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13100.12565513325199323No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12980.12450409386342534No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC12740.12220201508628958No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT11790.1130896199267939No Hit
CAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT11750.1127059401306046No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA11620.11145898079298941No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG11590.11117122094584744No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11560.11088346109870548No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA11500.11030794140442152No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAG10930.10484050430872412No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC25200.058.012832
GTGGTAT25300.057.7973941
GTATCAA79400.039.1887051
TATCAAC89750.035.2293472
GGTATCA43350.035.0945133
ATCAACG98600.032.0657463
TCAACGC98700.032.0332574
CAACGCA101600.031.2025765
AACGCAG102900.030.890976
ACGCAGA118500.026.8947437
CGCAGAG119000.026.7817388
AGTGGTA5600.026.5611062
CAGAGTA59500.026.0318510-11
GCAGAGT122600.026.0299859
GAGTACG64400.023.82022312-13
GTACTGG4250.023.004311
AGAGTAC57550.022.96357710-11
ATACGGG1754.3370164E-721.8612371
AGTACGG64000.020.48328812-13
ACGGGAT9600.020.36253516-17
Produced by FastQC (version 0.11.5)