FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1042536
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38990.37399188133551264No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT16750.16066591465426613No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT13530.1297796910610281No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT12190.11692641788868681No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11780.11299369997774657No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA11630.11155490074203672No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA11180.10723850303490719No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC10850.10407314471634552No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT16150.037.1408351
TGGTATC16400.036.3946572
CGGGTTA852.70702E-829.11942517
GTATCAA66350.027.3282741
TATCAAC77700.024.001062
GGTATCA32250.023.974211
AGAGTAC69850.023.7381911
CAGAGTA71450.023.47601110
TCAACGC78000.023.2969684
AACGCAG79150.023.272196
CAACGCA79750.023.2361075
ATCAACG78600.023.0885833
ACGCAGA82100.022.4035647
CGCAGAG80900.022.1931888
ACGGGTC4850.022.11475416
TGCCGAC5850.021.62629155
GCAGAGT83650.021.4656459
CCGAGAC650.003242008921.1510773
GAGTACG77800.020.78333712
ACGGGGG7600.020.6262616
Produced by FastQC (version 0.11.5)