FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505861
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15340.3032453579145259No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT8720.17237936903615816No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT7320.14470378226429792No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA6450.1275053819132133No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC6000.1186096575936868No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA5800.1146560023405639No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT5680.11228380918869016No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT5640.1114930781380656No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5590.11050466432478488No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC5290.10457418144510053No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAG5170.1022019882932268No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC6800.037.6043432
GTGGTAT7100.035.642261
CCGGTTG350.00410065731.4235979
GTATCAA28400.027.9907991
AACGCCC507.024809E-427.50109120
CGGCCGT400.00786751327.5010913
CCGTTAA400.00786751327.5010916
ACGGGTA1700.027.50108716
ACCGGTT400.00788271427.4902158
GGTATCA13550.026.1870291
GTCTATA651.0889527E-425.38062
TATCAAC34350.024.493942
ACTATCG1700.024.26326811
AGAGTAC30550.024.03294211
CAGAGTA31150.023.65596810
CCGAATT701.7981991E-423.57236114
ATCAACG35050.023.5317543
TCAACGC34950.023.2057654
CAACGCA35250.022.9257415
GCGCACG600.00203349522.91757219
Produced by FastQC (version 0.11.5)