FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120745
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT16910.1508817795305801No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15960.14240527506257No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT13650.12179398525088224No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC12510.11162217988927009No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT11380.1015396008904791No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT11320.10100424271355214No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9900.031.967151
TGGTATC10000.031.0665552
GTATCAA43100.029.2435051
GGTATCA19850.027.5888631
TATCAAC51250.024.7835062
AACGCAG52700.023.8897366
TCAACGC52550.023.7988744
CAACGCA52900.023.7983535
ATCAACG52850.023.6669463
AGAGTAC49850.023.60862711
CAGAGTA51550.023.3059810
ACGCAGA55500.022.6349587
CGCAGAG55700.022.4549798
GCAGAGT59800.020.9163579
ACGGGTA2500.020.90084816
AGTACGG56400.020.08946813
GAGTACG57300.020.06010412
GCCGACG3050.019.841355
TACGGGT9950.018.5183415
GTCCCGA950.001411481617.366811
Produced by FastQC (version 0.11.5)