FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1387182
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22220.16018085586462338No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9850.031.5548651
GTATCAA48700.029.256671
TGGTATC11500.027.7349622
GGTATCA21600.027.1237661
TATCAAC58000.025.078132
AACGCAG58650.024.1377646
TCAACGC58000.024.1247774
CAACGCA58700.024.1163375
AGAGTAC55550.024.05579411
ATCAACG58750.023.9571743
CAGAGTA56750.023.59387410
ACGCAGA61100.023.1698847
CGCAGAG62200.022.6717388
GCAGAGT65750.021.3260569
AGTACGG62000.020.7150813
GAGTACG63800.020.29940812
ACGGGTC3300.019.16862716
TACGGGT8900.018.85024315
TGCCGAC7150.018.84944255
TACGGGG24750.018.66857715
Produced by FastQC (version 0.11.5)