FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847087
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10970.129502636683127No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT8690.10258686533968767No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAT350.004097909731.43032616
GTATCAA30200.030.531921
GTGGTAT6600.027.941331
GGTATCA13900.027.5242961
TGGTATC6900.025.8995952
TATCAAC35750.025.8399642
GGGCCGT550.001229692624.9881116
CAACGCA37450.024.5863175
ATCAACG37250.024.5707513
AACGCAG37500.024.5549856
TCAACGC37500.024.4055064
AGAGTAC37350.023.48443211
ACGCAGA39300.023.3603887
CGCAGAG39550.023.2822238
CAGAGTA39000.022.7701910
GCAGAGT43650.021.1633179
CGGTTAA650.003243554621.1487839
AGTACGG41400.020.72579413
GAGTACG42250.020.4353912
TGCCGAC4050.020.37752555
Produced by FastQC (version 0.11.5)