FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975366
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18890.1936708886715346No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA14700.15071265555699093No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA10890.11165039585140346No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT10690.10959988353090021No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10450.10713926874629627No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC10320.10580643573796912No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35450.045.2095951
TGGTATC9550.044.5031552
GGTATCA17350.039.938671
TATCAAC42100.037.856752
GTGGTAT11450.037.4991461
TCAACGC43850.036.344074
ATCAACG44100.036.138043
CAACGCA44800.035.6682435
AACGCAG45350.035.2356646
CGCAGAG49000.032.69778
ACGCAGA49000.032.6109667
GCAGAGT52350.030.6864789
CAGAGTA39900.025.19011510-11
GTACTGG5350.023.8381731
AGAGTAC38600.022.79055610-11
TTCACTA3750.022.665788
TCACTAT3800.022.3675449
GAGTACG45050.022.16894312-13
AGTACGG43150.020.09191112-13
TAAGACT3203.6379788E-1219.9210954
Produced by FastQC (version 0.11.5)