FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975366
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18570.19039006895872934No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT11550.11841708650906428No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT9820.10068015493671094No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTA658.404095E-833.84034726
GTATCAA37300.030.4657121
TGGTATC8450.028.9610122
GTGGTAT8550.028.6413821
TATCAAC42700.026.723952
GGTATCA16250.026.5837571
ATCAACG43700.025.9183393
TCAACGC43850.025.8296784
CAACGCA44850.025.4963345
AACGCAG45200.025.238096
TGCCGAC4550.024.78740955
ACGCAGA46600.024.4798667
CGCAGAG46900.024.0888378
TCGCCCC801.604936E-524.06824538
AGAGTAC45750.023.85929511
CAGAGTA46650.023.75018910
CGATCGT600.002036733822.9139147
GCAGAGT50350.022.3848139
AGTACGG50250.021.01670813
GAGTACG52050.020.49705312
Produced by FastQC (version 0.11.5)