FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498015
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44830.9001736895475035No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT10350.20782506551007499No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT7650.15360983102918588No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA6910.13875084083812736No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT6800.136542072025943No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC6740.13533728903747877No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT6670.13393170888427056No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA6320.12690380811822938No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC6070.12188387899962852No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGAAAAGA5760.11565916689256348No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA5430.10903286045601036No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAG5380.10802887463229019No Hit
GCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGAATGGGGAAACCCACTTA5330.10702488880857003No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT5130.10300894551368936No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCC5100.10240655401945725No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA4990.10019778520727288No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31000.031.5121961
GTGGTAT5950.030.986311
TGGTATC5850.030.553712
GGTATCA13300.028.3452931
TATCAAC35450.027.8474042
CGGTAGA400.00784425827.5176931
CGGGACC1004.296453E-927.50386817
AGAGTAC35000.026.39310611
CAGAGTA35150.026.35869210
ATCAACG37850.025.638023
TCAACGC37950.025.4980264
AACGCAG38400.024.9844536
CAACGCA38850.024.9780985
CGGTACT901.4362449E-624.460171
ACGCAGA40050.023.9551327
CGCAGAG40150.023.8954688
GCAGAGT41700.023.2732969
AGTACGG38200.023.25121713
GAGTACG38650.022.97819512
CTGCGAG600.002032256222.9198920
Produced by FastQC (version 0.11.5)