FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622134
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19820.3185808845039815No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCG350.004097319731.42995317
ATCGCGT350.004097319731.42995318
TGGTATC5000.030.2464642
GTATCAA23700.029.8339791
CCGACGA852.701745E-829.12126555
GTGGTAT5250.027.774291
CGCGTTA400.007868716527.50120720
GGTATCA10450.027.1173551
TATCAAC27950.025.9719162
TCAACGC29200.024.663844
ATCAACG29100.024.6541333
CAACGCA29850.024.4049935
AACGCAG29800.024.2614446
AGAGTAC28250.024.23415611
CAGAGTA29150.023.6726710
CGCAGAG30500.023.3440978
ACGCAGA31100.023.24737
ACGGGAT4000.022.68849616
GCAGAGT31950.022.3725019
CGGGATT1605.4569682E-1122.3447317
Produced by FastQC (version 0.11.5)