FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083936
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41100.37917367815073955No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT30550.28184320845511174No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28910.266713163876834No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA19710.18183730404747145No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19310.17814704927228175No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC14960.138015528592094No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT14100.1300814808254362No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT14020.12934342987039826No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA13530.1248228677707909No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG12170.11227600153514598No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA11830.10913928497623476No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC11620.10720190121926018No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA11130.10268133911965283No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC14650.049.313712
GTGGTAT14950.048.6151281
GTATCAA50900.043.087211
TATCAAC59600.037.2203672
ATCAACG62300.035.607283
TCAACGC62400.035.480484
GGTATCA26300.035.3919831
CAACGCA63350.035.0154955
AACGCAG65100.034.074226
ACGCAGA71550.030.9996767
CGCAGAG72100.030.704278
GCAGAGT76000.029.1286549
CAGAGTA49650.024.04824410-11
GTATATA2053.4651748E-922.8063261
AGAGTAC47500.022.40837910-11
GAGTACG54000.021.9536512-13
AGTACGG52650.020.09542712-13
GCGTACA1306.233306E-419.6139142
TACGGGC7700.018.76223214-15
TTCACTA5050.018.5109048
Produced by FastQC (version 0.11.5)