FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083936
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70810.6532673515779529No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT16930.15619003335990317No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT13830.12759055885218315No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC12820.11827266554482921No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT11980.11052313051693088No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT11910.10987733593127269No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC11100.1024045700115136No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC9250.035.9685062
GTGGTAT9650.034.491911
GTCGCGC350.004093691731.4377251
GTATCAA46500.030.4067041
GGTATCA20850.027.5739771
TATCAAC55500.025.663482
TCAACGC56950.024.7123894
AGAGTAC54300.024.61020511
ATCAACG57250.024.5817573
CAACGCA57500.024.4748785
AACGCAG57650.024.4611326
CAGAGTA55650.024.15807310
ACGCAGA60300.023.386147
CGCAGAG60600.023.2250068
GCAGAGT62250.022.5673269
CGGGATA1901.8189894E-1221.7128417
AGTACGG61150.021.541613
GAGTACG62400.021.19626212
ACGGGAT8800.020.31466516
ACGGATA700.004976771319.6422322
Produced by FastQC (version 0.11.5)