FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931620
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22640.24301753934007428No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT15850.17013374551855906No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT11450.12290418840299694No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC10420.11184817844185399No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT10030.1076619222429746No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT10000.10733990253536849No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA9700.10411970545930745No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC9340.10025546896803418No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9100.030.5403771
TGGTATC9550.029.085672
GTATCAA41200.028.919121
GGTATCA19900.028.7610441
TATCAAC48000.025.1526872
TCAACGC49500.024.3231724
CGGGATA2150.024.3026117
ATCAACG49700.024.175173
AGAGTAC46300.024.11345911
CAACGCA50500.023.8985215
AACGCAG50650.023.7734686
CAGAGTA47400.023.61061110
ACGGGGG5150.023.4954216
GTACTGG3650.023.3702431
ACGCAGA52400.023.0319717
CGCAGAG52550.022.8615978
GCAGAGT54600.022.0067869
AGTACGG49750.022.0002613
GAGTACG50950.021.69799612
TGCCGAC4850.021.547855
Produced by FastQC (version 0.11.5)