FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191183
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT67810.5692660153813478No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47760.4009459503703461No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT33920.28475893292634297No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32670.2742651632872531No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA26130.21936176053553486No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT18720.15715469411501004No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC18310.15371273767338856No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT17620.14792017683261094No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA16310.13692270625084474No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG15770.13238939776675793No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA14750.12382648174126057No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC14350.12046847545675182No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAA13920.1168586187009049No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA13820.11601911712977771No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC13570.11392036320195974No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAG13500.11333271210217069No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA12380.10393029450554617No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTAT12240.1027549923059681No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC18900.052.84352
GTGGTAT19500.050.7944451
GTATCAA70050.042.662091
GGTATCA36700.038.8037451
TATCAAC80200.037.4124222
ATCAACG85100.034.9585953
TCAACGC85500.034.7950484
CAACGCA87250.034.194575
AACGCAG87850.033.961036
ACGCAGA96900.030.7853557
CGCAGAG97850.030.4877458
GCAGAGT102050.029.2329839
CAGAGTA60650.026.55568110-11
GTACTGG6000.026.214731
GAGTACG68550.023.06133812-13
AGAGTAC58550.022.46377810-11
ACAGGTA4750.021.4789261
GACAGGT3600.021.2551861
GTACGGA1455.4953434E-520.5222471
CGGTAGA1050.00418082920.2430341
Produced by FastQC (version 0.11.5)