FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191183
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG81430.6836061293688711No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT22160.18603354816178538No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT17140.14389056929120042No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC16440.1380140582933101No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT14380.12072032592808997No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA14300.12004872467118821No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA13660.1146759146159742No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC13520.11350061241639613No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT13420.11266111084526896No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAG12490.10485374623378607No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA12270.10300684277730625No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC12900.033.6721152
GTGGTAT12900.032.844481
GTATCAA58250.028.9059241
GGTATCA27550.027.5625171
GACGCCC400.0078669727.50449635
CGAGTCG400.007873422527.49987432
TATCAAC66750.025.7413792
ATCAACG70700.024.0279873
AGAGTAC65350.024.02416411
TCAACGC71100.023.8918084
AACGCAG71900.023.6661876
CAACGCA72300.023.6483295
CAGAGTA66850.023.4429910
ACGCAGA74500.022.8033567
CGCAGAG74400.022.760118
GACGTAT752.8760973E-421.99620428
GCAGAGT77950.021.6566759
AGTACGG72550.020.92428413
GAGTACG73900.020.87345912
GCCGTAT856.667317E-419.41901454
Produced by FastQC (version 0.11.5)