FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879715
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26290.2988467856067022No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA20020.22757370284694475No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17380.19756398379020476No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT15770.1792626020927232No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC15620.1775575044190448No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT15370.17471567496291412No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA14170.16107489357348687No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG13330.1515263466008878No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC13130.14925288303598325No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12250.13924964335040327No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA11840.13458904304234895No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAA11720.1332249649034062No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC11570.1315198672297278No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTAT11470.13038313544727556No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT11470.13038313544727556No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA11270.128109671882371No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAG10680.1214029543659026No Hit
GCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCTGAATGGGG10370.11787908584030055No Hit
ATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCA10070.11446889049294375No Hit
CCGCTACTGAGGGAATCTCAATTGATTTCTTTTCCTAAGGGTACTGAGAT9980.11344583188873669No Hit
GTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTC9950.11310481235400102No Hit
CTTAATGGGCTGATCCCCGTTCGCTCGCCGCTACTGAGGGAATCTCAATT9820.11162706103681305No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAG9440.10730748026349442No Hit
CGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGC8930.10151014817298784No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA8900.10116912863825216No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT11700.048.328421
TGGTATC11750.048.106352
GTATCAA45550.042.1877981
GGTATCA24850.041.573511
TATCAAC52550.036.7173842
ATCAACG54350.035.6536983
TCAACGC54700.035.5032544
CAACGCA56100.034.5415045
AACGCAG56850.034.160566
CGCAGAG60700.031.9238628
ACGCAGA60750.031.8975897
GCAGAGT63900.030.325179
CAGAGTA45850.026.69269610-11
GAGTACG51950.023.4357212-13
GACAGGT3900.022.8924061
AGTGGTA3400.022.4999852
AGAGTAC45100.021.71869310-11
GTACGGA1404.1889754E-521.2572361
TAGAACA1604.886053E-621.247574
GTACTGG4700.020.8049551
Produced by FastQC (version 0.11.5)