FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1385455
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43730.31563637938439No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA30880.22288706598193372No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27480.19834639161863793No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC21960.1585038850052871No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA20840.15041989815620138No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT20840.15041989815620138No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT19940.14392383729532898No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18730.13519024436015606No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG18730.13519024436015606No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC17290.12479654698276017No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC16950.1223424795464306No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA16200.11692909549570357No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA15940.11505245569145153No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAG15680.11317581588719951No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTAT15270.11021649927280208No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAA15180.10956689318671482No Hit
GTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTC15170.10949471473270514No Hit
ATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCA14130.10198815551569701No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC16800.045.791862
GTGGTAT17850.042.872491
GTATCAA63050.042.211781
GGTATCA32000.040.25661
TATCAAC70850.037.973692
ATCAACG75750.035.4586373
TCAACGC76050.035.4305344
CAACGCA78250.034.4344025
AACGCAG78800.034.301936
ACGCAGA85200.031.722977
CGCAGAG85600.031.574738
GCAGAGT93250.029.0755639
CAGAGTA59950.026.05100310-11
GTACTGG8050.025.8788281
GTCTAAG1452.072291E-623.4566731
GAGTACG67700.023.00602512-13
TTCACTA7000.021.85558
TACTGGT8200.021.7697982
AGAGTAC58950.021.66291810-11
CCGTCTT13250.019.5673984-85
Produced by FastQC (version 0.11.5)