FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1385455
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT26740.1930051860219206No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT19950.14399601574933868No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC18920.13656163498634022No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT18200.1313647862976423No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT17730.1279723989591867No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA17410.1256626884308765No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17150.12378604862662446No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC16320.1177952369438199No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA16270.11743434467377144No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA14680.10595797048623015No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCT14480.1045144014060363No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAG14220.10263776160178426No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCC14140.1020603339697067No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTCATCATGCGA14020.10119419252159038No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC12850.034.882512
GTGGTAT13800.033.1005061
GTATCAA62200.029.5523281
GGTATCA28150.027.5661661
TATCAAC70400.026.249432
ACTATCG4450.025.94980811
AACGCAG73850.024.7889926
ATCAACG73800.024.7694363
TCAACGC73950.024.6820224
CAACGCA75350.024.3311185
AGAGTAC72350.024.32127411
CAGAGTA72950.024.23255310
ACGCAGA75750.024.203517
CGCAGAG76450.023.945948
GCAGAGT80150.022.981019
AGTACGG79200.021.6669813
GAGTACG81250.021.28723112
TGCCGAC6050.020.9146855
CTATCGG5550.020.80885712
TATCGGT5950.019.41204513
Produced by FastQC (version 0.11.5)