FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1635807
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20550.1256260671338367No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT18880.11541703880714534No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57150.030.8164751
GTGGTAT13100.030.6690851
TGGTATC13300.030.1811072
TATCAAC65100.027.3248142
ATCAACG69050.025.315973
TCAACGC68950.025.3120524
GGTATCA25900.025.2869471
AACGCAG69800.025.125016
CAACGCA69650.025.099425
AGAGTAC67700.024.61185811
CAGAGTA68500.024.44110310
ACGCAGA72550.024.1347627
CGCAGAG74350.023.5504638
GCAGAGT77400.022.5876189
AGTACGG75500.021.71097613
GAGTACG78300.020.99906212
TCCTATA1459.722498E-920.8573272
GCGTTAC956.0309107E-520.2765311
ACGGGAT10000.019.52772516
CGAACTA2005.2750693E-1119.24680129
Produced by FastQC (version 0.11.5)