FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879483
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13770.15656925716585765No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT12190.13860415721509114No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT9740.11074688197497849No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC9250.10517542692695596No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39700.030.6328411
TGGTATC7850.029.4284552
GTGGTAT8200.028.5207271
GGTATCA17350.027.7519871
TATCAAC45800.026.7810252
TCAACGC47450.025.1456684
AACGCAG48050.025.0605376
ATGCGTA550.00122631825.00002541
TACCGTC550.001228759824.9914977
ATCAACG48150.024.9542313
CAACGCA48850.024.7626845
AGAGTAC46750.024.70450211
CAGAGTA47650.024.46872910
CGCAGAG50000.023.9195828
ACGCAGA50500.023.897817
GCAGAGT52500.022.8368199
TGCCGAC4350.022.7599455
AGTACGG53100.020.87101713
GAGTACG54000.020.777812
TACGGGC6800.020.62619615
Produced by FastQC (version 0.11.5)