FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641184
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT11180.1743649248889554No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC9700.15128262713979138No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT9300.14504416828866598No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9130.14239282327693767No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC7750.12087014024055498No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT7730.1205582172979987No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT7640.11915456405649548No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCT7570.11806283375754853No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA6990.10901706842341669No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCC6740.10511803164146329No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA6530.10184284074462246No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC6550.032.7438162
GTATCAA33400.032.608171
GTGGTAT7050.032.379241
TATCAAC39400.028.0547142
GGCGGAC400.00786061127.50714154
TCAACGC40500.026.807034
AACGCAG41300.026.6225976
AGAGTAC39250.026.61865211
ATCAACG40800.026.5446223
CAACGCA41950.026.4046065
CAGAGTA39900.026.39173910
GGTATCA13450.025.764751
ACGCAGA42700.025.685357
CGCAGAG42200.025.6659988
GCAGAGT44000.024.744849
CGGGGTC3500.023.5738717
AGTACGG43300.023.245413
AACTAGT600.002034251322.91725246
GAGTACG44650.022.78715912
ACGGGTC2550.022.64940516
Produced by FastQC (version 0.11.5)