FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153117
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA22220.19269510379259No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18620.16147537500531167No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT17610.15271650665110303No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC16520.14326386654606602No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA16400.1422232089198234No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT14630.1268735089327449No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC13530.11733414735885431No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC13100.11360512419815161No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA12910.11195741628993415No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTAT12720.11030970838171669No Hit
GTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTC12650.10970265809974182No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA12100.10493297731279654No Hit
ATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCA11760.1019844473717758No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG11650.10103051121438675No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12850.044.662161
GTATCAA47750.044.069781
TGGTATC12850.042.996812
GGTATCA24200.038.6470531
TATCAAC55300.038.4273872
ATCAACG59150.035.7090953
TCAACGC59150.035.7090954
CAACGCA59800.035.463095
AACGCAG61800.034.3841826
CGCAGAG64300.033.178078
ACGCAGA64500.033.075197
GCAGAGT68150.031.3660989
TATATAC2003.6379788E-1227.6242523
CAGAGTA54700.025.28843910-11
GTACTGG6300.024.2924331
GAGTACG61500.022.28501712-13
AGAGTAC53450.021.98395210-11
CGAGGTT1000.00315616221.2494263
TAGTACG1000.00315616221.2494264
ACGGGAT9600.020.3631516-17
Produced by FastQC (version 0.11.5)