FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824227
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28470.3454145520590808No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17410.2112282174692166No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA13120.159179449350725No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12390.1503226659645947No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC9850.11950591281285373No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT8940.10846526503014339No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT8560.10385488463736324No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC10150.053.602292
GTGGTAT10400.052.3264771
ACGCGCG250.007797498750.996659
GTATCAA37200.043.429571
TATACGT753.2623575E-739.666474
TATCAAC42200.038.2745932
GGTATCA18300.036.242521
ATCAACG45500.035.7745483
TCAACGC45950.035.516694
CAACGCA46700.034.946295
AACGCAG47600.034.196266
ACGCAGA53300.030.6171267
CGCAGAG53500.030.502678
GCAGAGT57200.028.4552979
TTGTACG600.006804649728.333193
CCGTGCG808.6126756E-426.5607579
CAGAGTA37850.024.86956810-11
CGTAGAC1401.5306923E-624.2855933
GAGTACG40500.022.92750712-13
AGAGTAC36200.022.48125810-11
Produced by FastQC (version 0.11.5)