FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824227
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT10570.12824137039917402No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9950.12071917081095379No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT8870.10761598443147337No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC8480.10288427823888321No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC7650.033.789322
GTGGTAT7750.031.9573151
CGGGCTA553.4878412E-530.00228717
GTATCAA34100.029.9398081
CCGGTTA507.027089E-427.50209250
TGCCGAC3450.025.51073655
TATCAAC41000.025.3525122
GGTATCA16100.025.2967851
TCAACGC40650.024.9513684
ATCAACG40700.024.9222283
AGAGTAC39050.024.71719211
CAACGCA42100.024.4822525
AACGCAG41900.024.4708866
CAGAGTA40300.024.28875710
CGCAGAG43100.023.7257848
ACGCAGA43650.023.4898097
TACGGGC5700.023.15965815
GGCGCGT600.002034142622.91841132
GCAGAGT46250.022.113899
AGTACGG43400.021.73552513
Produced by FastQC (version 0.11.5)