FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1243856
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT56570.454795410401204No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34830.2800163362961629No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25200.2025957988706088No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA23950.19254640408536036No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC17420.14004836572722243No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT16730.1345010998057653No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT15740.12654197913584853No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA15170.12195945511377522No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG13670.10990018137147707No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA13110.10539805250768577No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC12870.10346856870891807No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA12710.10218224617640627No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC12590.10121750427702242No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC17900.051.5205882
GTGGTAT17900.051.056011
GTATCAA60200.041.659781
TATCAAC69700.036.584122
GGTATCA31000.034.6912771
ATCAACG74150.034.503213
TCAACGC74600.034.4090164
CAACGCA77600.033.0240065
AACGCAG78600.032.711996
ACGCAGA87700.029.2668917
CGCAGAG88450.028.9706828
GCAGAGT92450.027.8102669
CAGAGTA54000.025.30136310-11
TATACCG900.001711483223.6103085
AGAGTAC52450.022.76761410-11
GAGTACG60900.022.33048212-13
GTACTGG6200.021.2535481
CTACGTC1455.5073724E-520.5165424
CCGTCTT14200.019.90369284-85
AGTACGG60600.019.74109512-13
Produced by FastQC (version 0.11.5)