FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1243856
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20950.16842785660076406No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT19450.15636858285846594No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT15750.1266223742941305No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT14150.11375914896901249No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC13960.11223164096165472No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT13130.10555884282424975No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC12400.032.589632
GTGGTAT12700.031.8351541
GTATCAA54950.029.1305481
GGTATCA26800.025.6565671
TATCAAC65000.025.1644462
AGAGTAC64350.024.2312311
TCAACGC67600.023.870294
AACGCAG68400.023.7939666
CAGAGTA65700.023.77518510
ATCAACG68200.023.6209323
CAACGCA69350.023.5048275
ACGGGGT10300.023.2295316
ACGCAGA70900.022.9937447
CGCAGAG71550.022.6320728
GCAGAGT74850.021.6394869
AGTACGG70550.021.36114513
GAGTACG72000.021.31290612
TGCCGAC5650.020.93212755
TACGGGG26300.020.18180315
TACGGGC9950.020.17707415
Produced by FastQC (version 0.11.5)