FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218410
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT63410.5204323667730895No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37990.31179980466345486No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26220.21519849640104727No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA18200.14937500512963617No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC13160.10800961909373692No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT12550.10300309419653483No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC18150.048.933182
GTGGTAT19750.044.3362241
GTATCAA61000.041.043471
TATCAAC69800.036.102152
GGTATCA33350.035.5604251
ATCAACG71600.035.194553
TCAACGC72550.034.675134
CAACGCA73500.034.284765
AACGCAG75350.033.3315736
ACGCAGA85700.029.3061167
CGCAGAG86100.029.1699668
GCAGAGT90950.027.7078979
CAGAGTA52000.025.70211810-11
CGTCAGA2152.3283064E-1023.7179185
GAGTACG56000.023.63810512-13
CTATACT3500.023.0684994
AGAGTAC49950.021.6948310-11
GTACTGG6900.020.9453871
ATTAGAC2101.1186421E-720.2355273
GTACGGG65350.020.19062814-15
Produced by FastQC (version 0.11.5)