FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903858
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT63880.7067481838961429No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39750.43978147009817914No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26280.2907536360800037No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA16740.18520608325644072No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC12330.13641523336630312No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT11230.12424518010572458No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT10560.116832511301554No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT10370.11473041119290862No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA10080.11152194260602881No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG9900.10953047934520689No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC9660.10687519499744429No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA9430.10433054749750514No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT16700.053.951361
TGGTATC16450.053.4735952
GTATCAA52700.039.35431
GGTATCA29350.034.6077041
TATCAAC60400.034.5445482
TCAACGC64300.032.252834
CAACGCA65150.031.8320335
ATCAACG65300.031.7571543
AACGCAG67200.030.9874466
ACGCAGA76150.027.2896467
CGCAGAG77200.026.918488
GCAGAGT81650.025.4513979
CAGAGTA42400.024.70574810-11
GAGTACG45500.022.83507512-13
TATACGG950.002343661622.3668795
TACATCG1152.7243263E-422.1723825
AGAGTAC41400.022.06912210-11
AGTACGG44650.020.27183512-13
GTACGGG54200.018.93445414-15
GTACTGG4750.018.7892171
Produced by FastQC (version 0.11.5)