FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences588233
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22280.378761477169761No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT10580.1798607014567357No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT8540.1451805662042082No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC7930.13481052576105046No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA7290.123930483328885No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC6910.11747045813478674No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA6610.11237043824470914No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCC6400.10880042432165485No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCT6330.10761041968063674No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT6200.10540041106160314No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT6180.10506040973559798No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC6100.032.8911172
GTGGTAT6150.031.7434021
GTATCGT350.004107740231.4133646
GTATCAA29200.030.7918031
CGACGCT400.00786251427.50540451
GGTATCA13300.027.4960441
ACAACGC1103.9835868E-1027.4796834
TATCAAC34800.026.7735542
AGAGTAC33100.025.75596411
AACGCAG36550.025.4938136
CAGAGTA33700.025.29524810
TTAGGCC1101.1978045E-824.9815314
TCAACGC36300.024.9815314
CAACGCA37450.024.9524215
CGGGATA1551.8189894E-1224.84358817
ATCAACG36900.024.64983
ACGCAGA37700.024.6432427
CGCAGAG38700.023.793398
GCAGAGT39850.022.9056749
AACAACG1354.276444E-922.3908543
Produced by FastQC (version 0.11.5)