FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences869659
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20960.24101400663938394No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT18470.21238209459109836No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT14590.16776690633915134No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC13480.15500328289593968No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC12350.14200968425555305No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA12120.13936496948804072No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT11580.13315563916431614No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA11390.13097087479115377No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT10930.12568144525612915No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCC10610.12200184210132937No Hit
GTATCAACGCAGAGTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCT10210.11740233815782967No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAG10190.1171723629606547No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGAAAAGA9950.11441266059455488No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA9230.10613355349625543No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA9140.10509866510896801No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTCATCATGCGA9060.10417876432026806No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC7800.035.602792
GTGGTAT8500.033.3311651
GTATCAA39250.030.8350871
GGTATCA17250.028.2238541
TATCAAC44950.027.4646432
TCAACGC47250.026.0068724
TGCCGAC3600.025.97966655
AGAGTAC45100.025.97259511
ATCAACG47600.025.8171273
CAACGCA48150.025.5778565
CAGAGTA46450.025.45306410
AACGCAG48250.025.3553736
CGCAGAG49700.024.6156318
ACGCAGA50450.024.2496917
ACGGGTA2950.024.23865516
GCGCAAT801.6090924E-524.05970611
CGGGATT2800.023.5727617
GCAGAGT52350.023.4772999
GATCGAC1302.744855E-923.26920720
AGTACGG50050.022.91074613
Produced by FastQC (version 0.11.5)