FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288494
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT65600.5091215015359016No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52940.4108672605382718No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34160.26511570872662193No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT27430.21288418882819787No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13500.10477347973680902No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT16850.048.6937561
TGGTATC17500.047.6046752
GTATCAA63200.043.4541551
TATCAAC71450.038.4292452
GGTATCA31950.037.7888221
TCAACGC76200.036.025334
ATCAACG77350.035.4911043
CAACGCA77850.035.3163765
AACGCAG81800.033.7148976
CGCAGAG89400.030.7061548
ACGCAGA91300.030.2067747
GCAGAGT92650.029.6301889
GACAGGT5600.027.3294141
CAGAGTA53100.026.12979310-11
GAGTACG57750.023.87866412-13
AGAGTAC50400.023.31373610-11
GTACGGG62350.022.04880714-15
TAGCGTG1000.003158331621.2471375
AGTACGG55750.021.00022912-13
ACGGGAT11600.019.41548716-17
Produced by FastQC (version 0.11.5)