FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1467229
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT79440.5414287749219788No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61040.41602231144558893No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39910.27200934550775646No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA20270.1381515768840447No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCT18490.1260198646564374No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17870.12179421208277645No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC18500.058.3467182
GTGGTAT19050.056.675691
GTATCAA72750.040.6662671
TATCAAC83000.035.994132
GGTATCA39000.035.5312961
TCAACGC86350.034.6457524
CAACGCA87650.034.1803785
ATCAACG88100.034.0540243
AACGCAG91650.032.7349626
CGCAGAG99250.030.2283068
ACGCAGA99500.030.1950667
GCAGAGT107000.028.158029
CAGAGTA60850.025.0361910-11
AGAGTAC56850.022.64914510-11
GAGTACG71350.020.99446512-13
GTACGGG72100.020.45260814-15
GCTACGA1050.00418912220.2364882
GACAGGT7000.020.0389041
AGTACGG66050.019.33353612-13
ACGGGAT12250.019.25357216-17
Produced by FastQC (version 0.11.5)