FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1467229
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31190.2125775867298152No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCT19780.1348119482371191No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGAC17620.12009031991597767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC9950.038.6990362
GTGGTAT10450.037.1333961
GTATCAA61600.030.5141891
GGTATCA26550.028.5055891
TATCAAC69500.027.147792
AGAGTAC65700.026.0325411
TCAACGC72700.025.9008754
CAACGCA74050.025.7256555
ATCAACG73900.025.5209673
AACGCAG75650.025.2178926
CAGAGTA70350.024.85735510
ACGCAGA78050.024.478517
CGCAGAG77750.024.3961738
AGTACGG73450.023.02682513
GCAGAGT83150.022.8479949
CGATAGG852.5735097E-522.6441422
ACGGGAT14100.021.65131216
GAGTACG78150.021.60457412
TACGGGG28800.021.58222815
CGGGATA3500.020.43081517
Produced by FastQC (version 0.11.5)