FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614552
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30430.49515744802718076No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20150.32788112315963497No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14250.23187622853721085No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA8990.14628542417891408No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT8520.13863757664119555No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC7030.11439227274502402No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT6320.10283914135825772No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8350.048.355331
TGGTATC8650.047.165782
GTATCAA30350.040.8913421
GGTATCA15150.037.8728831
TATCAAC34750.036.3223152
ATCAACG36600.034.3702353
TCAACGC36750.034.1143074
CAACGCA37250.033.65645
AACGCAG38050.032.9487726
ACGCAGA43150.029.054487
CGCAGAG44000.028.3966128
CTAGACT600.006804459728.3322224
GCAGAGT46650.026.9657149
GTATTAG2550.026.6677911
CAGAGTA26100.025.5641310-11
ACGGGAT4350.023.93584316-17
GTAATAC1601.829103E-723.9053133
GAGTACG28450.023.22843712-13
ATACTAC1152.7209055E-422.1748471
GTATTAT1353.1695734E-522.0379641
Produced by FastQC (version 0.11.5)