FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614552
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19230.3129108684049519No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT7740.1259454041317903No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT6310.10267642119788073No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC6100.032.8990752
GTGGTAT6000.032.0963481
TAGTCGG350.004098191431.42852430
GTATCAA27500.029.7120481
CGGGATA1256.002665E-1126.4021117
TATCAAC31850.026.2394542
CGGGATT1500.025.66871817
TCAACGC32050.025.471144
ACGGGAT4650.025.43214216
CAGAGTA29400.025.24889810
AGAGTAC29200.025.2355811
AACGCAG32200.025.183816
CAACGCA32550.025.079885
ATCAACG32550.025.079883
GGTATCA12500.024.2098181
ACGCAGA33700.024.0648297
CGCAGAG33800.023.9122968
ACGGGTC1453.9108272E-1022.7604416
GCAGAGT36100.022.4649529
AGTACGG32450.022.20516413
Produced by FastQC (version 0.11.5)