FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128257
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21890.19401607966979156No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT16100.1426979845903903No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC12790.11336069707522312No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT12130.10751096602990276No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC11000.035.006832
GTGGTAT11400.034.5299031
GTATCAA52450.029.7578641
GGTATCA24650.027.0248091
TATCAAC62600.025.2645132
CGGGGCG801.608528E-524.0618617
ATCAACG62950.024.0242373
AGAGTAC56950.023.99848411
TCAACGC63050.023.9840074
CAACGCA63850.023.8966875
CAGAGTA58150.023.8783910
AACGCAG63500.023.8119326
TAACGGC952.31378E-623.1603636
ACGCAGA65900.022.9874637
CGCAGAG65800.022.897058
TTAACGG852.5681538E-522.6494735
GCAGAGT69200.021.8137159
AGTACGG62500.021.69238513
GAGTACG63750.021.22390612
AACGGCC1207.5842763E-720.62719537
Produced by FastQC (version 0.11.5)