FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974351
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12120.12439049172218225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT19200.038.3812561
TGGTATC19200.038.3595852
GTATCAA59650.025.3995951
CCGATCG901.4448142E-624.44807446
AGAGTAC47200.023.88880511
CAGAGTA47700.023.81136310
GGTATCA32250.023.2610173
AGTCCGG852.5648153E-522.65274854
TATCAAC66850.022.6100562
TCAACGC71300.021.0811164
ATCAACG71700.021.078483
AACGCAG71900.020.9062676
CAACGCA72800.020.6090055
GTCCGGA956.0514605E-520.26616755
AGTCCGC956.056247E-520.26408621
CGGGATA2850.020.26304417
GCTTGTA3000.020.1644821
GCCCGTC700.0049756619.64274815
GCGCGCC856.680842E-419.41265111
GATCGTA1452.2048334E-718.97223348
Produced by FastQC (version 0.11.5)