FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences946866
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT34040.3595017668814806No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23060.24354026863357645No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT16830.17774426370785307No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16600.17531519771541063No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC9550.054.7462462
GTGGTAT10800.049.2022251
GTATCAA40700.042.4062771
GGTATCA22000.038.6461141
TATCAAC45600.037.7522472
ATCAACG49200.035.0707243
TCAACGC49400.034.9287384
CAACGCA50350.034.3559275
AACGCAG53200.032.595326
CGCAGAG56150.030.72828
ACGCAGA57900.029.8728527
GCAGAGT58550.029.5412169
CAGAGTA37950.025.6999210-11
GAGTACG43750.022.34023512-13
AGAGTAC36400.022.00750710-11
CTATCGG1857.3946467E-720.6808931
GTACGGG47600.020.62366714-15
TACTATA1455.4979377E-520.520252
ACGGGAT8000.020.45180316-17
ATACGGC1050.004185122420.2389266
Produced by FastQC (version 0.11.5)