FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1979749
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85400.431367814808847No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT64350.32504120471837594No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48310.2440208329439742No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT35820.1809320272418372No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32630.1648188735036613No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT24300.1227428325509951No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21620.10920576295277837No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT31650.057.765531
TGGTATC31800.057.1996272
GTATCAA119500.038.8889581
GGTATCA62750.038.418761
TATCAAC135950.034.35542
ATCAACG144950.032.13433
TCAACGC144950.032.104984
CAACGCA149000.031.3749455
AACGCAG151600.030.8368536
GTCGAGG22600.028.5839778
CGCAGAG171500.027.2593868
ACGCAGA171800.027.1863577
TCGAGGT23750.026.4854629
GACAGGT10150.026.3905541
GCAGAGT180350.026.017319
AGTCGAG32250.025.1698257
CAGAGTA89500.024.50456810-11
AGAGTAC83650.022.28045510-11
GAGTACG100900.021.48322112-13
GTACGGG109800.019.62549214-15
Produced by FastQC (version 0.11.5)